THE PROTECTIVE EFFECT OF DAFLON AGAINST THE RENAL-HEPATIC TOXICITY INDUCED BY METHOTREXATE IN RABBITS

Abstract

Methotrexate (MTX) is considered a folic acid antagonist, used frequently in the treatment of various types of diseases and has had a major role in the treatment of inflammation and suppressive material for immunity in Non – tumor diseases like acute arthritis and psoriasis. This study was designed to investigate the effect of methotrexate and pre-treatment with daflon in attenuation of its development for renal-hepatic toxicity. Twenty-four healthy, local, domestic rabbits were randomized divided into 4 groups: group 1 (control), group 2 (20mg\kg MTX group), group 3 (Daflon 100 + MTX) and group 4 (Daflon 200 + MTX). At the end of experiment, blood samples were collected from the heart then the liver and kidney tissues were excised. Plasma level of S.ALT, S.AST, ALP, total protein, bilirubin, urea, creatinine and oxidation parameters (GSH, and MDA) were measured. The Results show that the Compared with the control group, levels of S.ALT, S.AST, ALP, urea, creatinine and MDA were increased (p<0.05), while total protein and GSH were decreased (p<0.05) in MTX group with deterioration of liver and kidney tissues. While group 3 (Daflon 100 + MTX) and group 4 (Daflon 200 + MTX) showed decreased levels of S.ALT, S.AST, ALP, urea, creatinine and MDA while total protein and GSH were increased (p<0.05) in as compared with MTX group with improvement of liver and kidney structures. Conclusions: The results of the present study reveal that daflon has been shown to attenuate MTX induced hepato-renal toxicity.