EVALUATION OF THE ANTIBACTERIAL ACTIVITY OF SOME MEDICINAL PLANTS EXTRACT AGAINST SOME HUMAN PATHOGENIC BACTERIA

Abstract

The need of effective and economical antibiotics and antimicrobials has led to search for novel sources such as medicinal plants, fungi, archaea etc. In the present study, Daphne mucronata and Mallotus philippensis methanolic extracts were screened as antibacterial agents compared to some antibiotics against eight local isolates of human pathogenic bacteria. The susceptibility test was performed through well diffusion and disc diffusion method. Results reported that D. mucronata showed significant antibacterial activity against all the selected strains. The highest zones of inhibition of D. mucronata seed extract were recorded against, Providencia stuartii (20mm), Brucella abortus (19.3mm), Proteus vulgaris (19.3mm), Klebsiella pneumonia (19mm), followed by Pseudomonas aeruginosa (19mm), Entero-bacter sakazaki (18.3mm), Escherichia coli (16.6mm) and Staphylococcus aureus (16.6mm). D. mucronata seed extract was reported as the most active against B. abortus (19.3mm), E. sakazaki (18.3mm), E. coli (16.6mm) and K. pneumoniae (19mm) when compared to Cefepime (8.1mm) and Ciprofloxacin (14.2mm). However, D. mucronata methanolic seed extract showed about similar antibacterial activity against P. vulgaris (19.3mm), P. stuartii (20mm), P. aeruginosa (19mm) and S. aureus (18.7mm), compared to the following antibiotics Cefepime (19.3mm) Ciprofloxacin (21mm), Moxifloxacin (21mm) and Ceftriaxone (20mm). On the other hand, M. philippensis showed insignificant antibacterial activity against the selected bacterial strains, but not significant compared to selected antibiotics. As conclusion, D. mucronata extract was considered as high potential antibacterial and recommend to be used as novel and economical source of effective antibacterial agent to combat against human pathogenic bacteria.