DETERMINATION OF DNA FINGERPRINTING OF NINE ARGEMONE PLANT SAMPLES USING RAPD-PCR TOOL

Abstract

We are herein focusing on molecular analysis of Argemone plants collected from open
area (Al-Shafa, Jabajeb and Al-Arafah), dams (Gadeer Albanat and Ekrima) and valleys
(Thumalah, Wadi-Sa'b, Saysid and Wadi-Jaleel) at Taif Governorate in Kingdom of Saudi Arabia.
At the level of molecular analyses, DNA fingerprinting of nine Argemone plant samples (1-9) via
RAPD-PCR using 10 random primers was determined. Data showed that total fragments of 108 (28
polymorphic fragments+80 monomorphic fragments) were amplified. Out of the 108 fragments,
PS21 samples showed 84, 82, 84, 84, 83, 87, 85, 85 and 83, respectively. A number of 14 unique
fragments out of the 108 were obtained using the ten RAPD-PCR primers, and could be used as
DNA markers. The dendrogram based on RAPD-PCR analysis of the nine PS21 samples showed
the presence of four clusters (A, B, C and D) with highest and lowest similarities of 98 and 89 %,
respectively. Results of SDS-PAGE analysis of the applied PS21 samples was in harmony with that
of RAPD-PCR analysis. Results paid an attention to the availability of RAPD-PCR technique as
one of the molecular tool for determining the DNA fingerprinting of such important plants.