NESTED-PCR ISOLATION OF BAR GENE FROM A RESISTANT-BASTA HERBICIDE STREPTOMYCES COELICOLOR EGY

Abstract

Resistance of Streptomyces isolate against Basta (Bialophos) herbicide was tested on three
doses of Basta (2, 4 and 6g/L) in starch nitrate agar medium (SNAM). The stressed cultures were
studied to find gene confers resistance for herbicide. The bar gene was isolated via polymerase
chain reaction (PCR) from the culture. This local isolate was identified as Streptomyces coelicolor
Egy. Almost 450pb amplified DNA fragment was cloned at ELORI and Hind III sites in plasmid
pAHC25 with gus genes. The construct was transformed into coli strain DH5α.