USE OF CHEAP MEDIA TO ENHANCE GLUCOSE OXIDASE PRODUCTION DURING BATCH CULTIVATION OF ASPERGILLUS NIGER

Abstract

The study was carried out to check the parameters affecting glucose oxidase
production, optimizing the growth culture conditions and to establish a cost
effective medium for industrial production of this enzyme. In this study,
Aspergillus niger PTTC 5012 strain was grown on different simple and complex
sources of carbon and nitrogen in shaking flasks. Different culture conditions were
also applied to find optimal values of pH, temperature, CaCO3 concentration and
culture time for growth and enzyme production. Beet molasses and corn steep
liquor showed to be very high promising carbon and nitrogen sources for glucose
oxidase production. Using 50 g l-1 molasses and 10 g l-1 corn steep liquor and at
optimal conditions of pH 5.5, temperature 30 oC, CaCO3 concentration of 50 g l-1,
culture time of 72 h and with previously optimized conditions of oxygenation, the
activity of glucose oxidase reached to around 550 U ml-1. The results of the
present study could be scaled-up for a commercial production of glucose oxidase
at a satisfactory productivity level using molasses and corn steep liquor as the
basal medium.