CHEMILUMINSCENT AND COLORIMETRIC TECHNIQUES FOR THE DETECTION OF BANANA BUNCHY TOP VIRUS

Authors

  • Allam, E.K., J.L. Dale, Sohair I. El-Afifi, R.M. Harding and A.S. Sadik

Abstract

The most important virus disease of banana is bunchy top disease (BBTD) caused by
banana bunchy top virus (BBTV). Some non-radioactive techniques were developed such as
chemiluminscent and colorimetric assays in routine detection of BBTV in the field. Comparison
of radioactive and non-radioactive detection techniques of BBTV was also studied. Results
showed that BBTV was completely detected in Southern blot and dot blot hybridizations after 20
min -6 hours and 3-24 hours from hybridization with pBT338 insert labelled with digoxigenin
(11-dUTP) using AMPDD and NBT X-phosphatase, respectively. This result strongly
recommends the use of non-radioactive techniques, i.e., chemiluminscent and colorimetric
techniques for the detection of BBTV and other plant viruses.

Published

2021-03-28

How to Cite

Allam, E.K., J.L. Dale, Sohair I. El-Afifi, R.M. Harding and A.S. Sadik. (2021). CHEMILUMINSCENT AND COLORIMETRIC TECHNIQUES FOR THE DETECTION OF BANANA BUNCHY TOP VIRUS. Pakistan Journal of Biotechnology, 5((1-2), 101–107. Retrieved from https://pjbt.org/index.php/pjbt/article/view/221

Issue

Vol. 5 No. (1-2) (2008): Pak. J. Biotechnology

Section

Research Articles