ANTIHYPERTENSIVE PEPTIDES PRODUCED BY INDIGENOUS LACTIC ACID BACTERIA FROM DADIH ORIGIN

Abstract

In this study the capability of Lactobacillus plantarum ssp. plantarum DG17 and Lactococcus lactis ssp. lactis DK12 isolated from dadih origin was considered to generate antihypertensive peptides in reconstituted skim milk (RSM) medium followed bymeasuringangiotensin converting enzyme (ACE) inhibitor activity, protein profile and protein sequence. Theisolates showed high ACE inhibitor abilities i.e. 60.79±6.2% and 61.98±7.8%, with IC50 values of 439.9 μg/mL and 442.2 μg/mL respectively. Two smaller molecular weight bands ranging in size from 12 to 14 kDa were recorded in peptides profile of these isolates and assumed as antihypertensive peptides. The peptides sequence with molecule weight less than 3.0kDa were identified by LC Nano/MS and then compared withthe peptide database (Milk Bioactive Peptide Databaseand BIOPEP-UWM).One of the peptide sequence (VVVPPF) generated from Lactobacillus plantarum ssp. plantarumDG17had high ACE inhibitor activity due to the presence of F residue at the C terminal and V residue at the N terminal. The experimental result indicated that Lactococcus lactis ssp. Lactis DK12 and Lactobacillus plantarum ssp. plantarum DG17 isolates were potential developed into a starter culture in functional fermented milk.