REDUCTION IN BANANA BUNCHY TOP VIRUS INFUTION RATE BY USING STREPTOMYCES CHIBAENSIS FILTRATE

Abstract

Banana bunchy top virus (BBTV) is one of the most important viral agent affecting
banana production worldwide. The present study aimed at performing a trial to
control BBTV biologically by using Streptomyces chibaensis, via induction of the
plant resistance either as a cell-free filtrate or spore suspension before and after virus
inoculation. Various morphological and physiological measurements were estimated.
Also enzyme-linked immunosrobent assay, e.i., ELISA and polymerase chain
reaction, e.i., PCR were employed in virus indexing among banana plants under
study. The viral infection in plants with pre-inoculated through the cell-free filtrate
was disappeared, while the other treatments didn't acquire any resistance against
BBTV. The cell-free filtrate of S. chibaensis having antagonistic agent(s) as a
secondary product(s) that inhibit BBTV infection, or this filtrate is inducing the plant
resistance. Two filtrate may contain chitinase enzyme that could be in developing
resistance against BBTV either through two inhibition if biting of aphids (Pentalonia
nigronervosa) to banana. As it is a ruler for BBTV.