ESTABLISHMENT OF REGENERATION SYSTEM VIA ORGANOGENSIS IN SOME EGYPTIAN CULTIVARS OF VICIA FABA

Abstract

Faba bean (Vicia faba L.) is one of the most important legume crops worldwide particularly in
Egypt. The present investigation was designed to establish the regeneration system of two cultivars of
faba bean (cv. G 461 and G 674). The regeneration system was carried out via organogenesis using
shoot apices and cotyledon explants obtained from the two cultivars used. The VSI medium (MS + B5
vitamins) containing different concentrations of BAP and NAA for callus as well as shoot induction was
used and the cotyledon explants appeared to be more effective than shoot apex explants. After 3 weeks
from culturing at 28±2oC and photoperiod 16/8 h light/dark, the VSI medium supplemented with 2 mg/L
BAP and 1 mg/L NAA or 3 mg/L BAP and 0.05 mg/L NAA was successful for producing calli for the
two cultivars, respectively. The VSI medium combination with 5 mg/l BAP and 0.5 mg/L NAA and 3
mg/L BAP and 0.05 mg/L NAA were the best media for shoot induction using the shoot apex explants
from the two cultivars (G 461 and G 674), respectively. Six different media were used for root induction
and the M4 medium (B5 medium free hormones) was the most successful one