INTRACELLULAR INVERTASE AND SUCROSE HYDROLYSIS BY CALCIUM ALGINATE ENTRAPPED MUTANT CELLS OF SACCHAROMYCES CEREVISIAE NA-47

Abstract

Biocatalyst by entrapping the whole mutant yeast cells of Saccharomyces cerevisiae NA-47 into
calcium-alginate for the production of inverted syrup (glucose and sucrose) from sucrose as
substrate. Out of M1-M8, the medium M2 containing glucose (2.0 %) as carbon source was selected
for cell growth and intracellular invertase after the time interval of 48 h. The optimum conditions
for sucrose hydrolysis were as sucrose (50 %), alginate beads were repeatedly used of 26 days after
every 18 h of incubation time. The beads were also stored at 4°C for 6 months without appreciable
loss of the invertase activity