INFLUENCE OF DIFFERENT CARBON SOURCES ON THE PRODUCTION OF ALPHA AMYLASE BY ASPERGILLUS ORYZAE ON KINETIC BASIS

Abstract

The present study is concerned with the selection of carbon source for the production of
alpha amylase by Aspergillus oryzae. Different carbon sources such as the lactose, glucose,
maltose, xylose and sucrose were tested for the production of enzyme. Of all the
carbohydrates tested lactose at the level of 1.0 % gave the maximum production of alpha
amylase (130 U/ml) after 72 h of inoculation. The kinetic analysis indicated that the
volumetric production of biomass and alpha amylase was significantly higher in the presence
of lactose added to the fermentation medium.