ESTABLISHMENT OF REGENERATION AND TRANSFORMATION SYSTEMS OF F144 SUGARCANE CULTIVAR

Abstract

Sugarcane is one of the most important (two sugar) crops for sugar production in
Egypt. In this study, an experiment was conducted to establishment the regeneration system
for sugarcane., F144 using leaf bases as a explants through somatic embryogenesis. The
effect of 2,4-D, NAA, IAA and kinetin hormones on the sugarcane regeneration was studied.
The microprojectile-mediated method was used to transform plasmid pAB6 carrying the gus
and bar genes as reporter and selectable markers respectively. The presence of the transgenes
was confirmed by PCR analysis through which amplified fragments with sizes of
about 1800(gus) and 540 bp (bar) were detected in transgenics. GUS assay and leaf painting
detect the expression of both gus and bar genes.