MHC I MOLECULAR MARKER INHERITANCE AND FIRST GENERATION CATFISH (CLARIAS SP.) RESISTANCE AGAINST AEROMONAS HYDROPHILA INFECTION

Abstract

Three groups of A. hydrophila-challenged sangkuriang catfish strain were obtained, namely: resistant (R: no
injuries and survive), recover from injuries and survive (L), and dead fish. In the present study, the inheritance
of the major histocompatibility complex (MHC) 1 marker in the first generation (F1) of catfish and their
resistance to A. hydrophila infection were evaluated. The F1 offsprings were produced by crossing female R and
male R (abbreviated:RxR), LxL, RxL, LxR, and control fish (C) each was replicated twice (two brood pairs per
crossing). Results of the PCR analysis using ClMHAh-01 primer showed that the percentage of F1 progenies
from R and L broods carrying MHC I marker ranged between 62.5 and 83.4%. The challenge test with A.
hydrophila 106 cfu/mL showed that the survival of F1 fish (76.8-87.0%) was more two times higher than that of
C fish. Blood profiles and antibody titer were in line with high resistance of F1 offsprings from R and L broods
against A. hydrophila infection. All the results indicated that A. hydrophila resistant catfish can be produced by
MHC I marker based selection.