OPTIMIZATION OF PRODUCTION OF PROTEASE BY LACTOBACILLUS PLANTARUM SK (5) FROM BEKASAM WITH RESPONSE SURFACE METHODOLOGY

Abstract

Lactobacillus plantarum SK (5) is one of lactic acid bacteria isolated from Indonesia fermented fish know
as bekasam. The isolate was chosen to optimize its protease production by using response surface methodology.
The aim of this study was to select the composition of medium having the highest specific protease activity
produced from L. plantarum SK (5). The variables involved in this study were glucose, peptone, yeast extract,
volume of inoculant, and pH. These variables would be optimized by central composite design (CCD) matrix of
response surface methodology. The optimal cultural condition for protease production obtained with response
surface methodology were glucose 6%, peptone 6%, yeast extract 7.5%, volume of inoculant 3 mL and pH 6.0.
The specific protease activity under unoptimized conditions was 3.615 U/mg protein. Under the final optimized
conditions, the predicted response from protease production was 6.393 U/mg protein and the observed validated
experiment value was 6.503U/mg protein. The optimization of the production of protease with response surface
methodology resulted in about two folds increase in the production of protease by L. plantarum SK (5).