STUDIES ON IN VITRO SURFACE STERILIZATION AND ANTIOXIDANTS ON GUAVA SHOOT TIPS AND NODAL EXPLANTS

Authors

  • Roshan Zamir
  • Syed Tariq Shah
  • Nawab Ali
  • G.S.S. Khattak
  • T. Muhammad

Abstract

In vitro studies were carried out to investigate the effect of different surface
sterilization agents and antioxidants on the guava (Psidium guajava L.) Cv. Safeda shoots
tips and nodal explants. The best sterilant observed for shoot tips was mercuric chloride
(HgCl2) at 0.05% for 5 minutes plus 70% ethanol which gave maximum survival
percentage of (67%) while minimum were survival (22%) was observed with 2% Sodium
hypochlorite for 10 minutes. In case of nodal explants the best sterilant was 4% Calcium
Hypochlorite (CaOCl2) for 10 minutes which gave 25% survival.
Blackening of media was reduced and gave 55% green shoot tips when 75:50mg/l
citric acid and ascorbic acid was supplemented to the medium. While 75:50 mg/l citric
acid and ascorbic acid, 100mg/l Polyvinyl pyrolidone (PVP) and 200mg/l Activated
charcoal when added to the medium gave 13% green nodal explants.

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Published

2004-12-25

How to Cite

Zamir, R. ., Syed Tariq Shah, Nawab Ali, G.S.S. Khattak, & T. Muhammad. (2004). STUDIES ON IN VITRO SURFACE STERILIZATION AND ANTIOXIDANTS ON GUAVA SHOOT TIPS AND NODAL EXPLANTS. Pakistan Journal of Biotechnology, 1(2), 12–16. Retrieved from https://pjbt.org/index.php/pjbt/article/view/148

Issue

Vol. 1 No. 2 (2004): Pak. J. Biotechnology

Section

Research Articles