BIOSYNTHESIS OF ALPHA AMYLASE FROM ASPERGILLUS FUMIGATUS (FRESENIUS 1863) IN SUBMERGED FERMENTATION

Abstract

In the present work optimization of some parameters for alpha amylase production by
Aspergillus fumigatus (Fresenius 1863) in submerged fermentation were studied. Various agricultural based
by-products (sunflower waste, cotton stalk, rice husk, date syrup and molasses) were used as sources of carbon.
Optimal conditions for the production of α-amylase (7.01 U/mL) by A. Fumigates were observed when the
strainwas grownonculture medium (M1) containing casein as a source of nitrogen, molasses as a source of
carbon after 72 h of incubation at initial pH 5.5, temperature 35° C, inoculum size of 6x106 conidia in 50 mL of
culture medium and agitation rate of 150 rev/min.The strain was proved thermo (up to 60° C) and pH (up to 9.0)
stable so it might be a potential strain for industrial utilization