UP TAKE OF GLYCOLIPIDS BY MYCOPLASMA CAPRICOLUM MEBRANES

Abstract

Mycoplasma capricolum was grown with and without DGDG containing cholesterol on Edwards modified media. Total lipids were extracted with chloroform: methanol (2:1). Membrane protein and phospholipids were determined by Lowery and Chen methods respectively. Total lipids were separated on TLC and glycolipids were located with iodine while diphenylamine and orcinol test was performed for lipid sugar. Three positive sugar spots for microorganisms grown on DGDG and cholesterol were found. Either DGDG is being metabolized to new components or new glycolipids are being synthesized from DGDG.