KINETRIC ANALYSIS F NUTRITIONAL STRATEGIES FOR NVERTASE PRODUCTION BY SACCHAROMYCES CEREVISIAE KR18

Abstract

Fermentation conditions for invertase secretion by Saccharomyces cerevisiae strain were optimized. Submerged fermentation technique was employed for present investigation. 6.76±0.5 U of invertase ml-1 of fermented broth were obtained by Saccharomyces cerevisiae KR18 when fermentation medium was supplemented by 15.0 mg sucrose ml-1 and incubated at 25oC at initial pH 6.0. Sugar
consumption and cell mass production (mg ml-1) at this pH level were 11.21±0.5 and 4.67±0.5, respectively. One ml of vegetative  noculum (1.2 × 103 cells) developed for 12 h was used. Kinetic analysis of fermentation results was made to check out the feasibility of fermentation process. All the kinetic parameters i.e. product (Yp/x, Yp/s) and growth rate (Yx/s) coefficients as well as specific rates (μ h-1) were
favorable for optimized conditions.