MASS PRODUCTION OF BANANA (MUSA SP.) THROUGH BIOTECHNOLOGICAL TECHNIQUES

Abstract

Meristematic tip with leaf primordial from 13 clones of desert banana (Musa spp.) were evaluated for in-vitro propagation techniques accelerates the production of disease-free banana (Musa spp.) clones through shoot tips. It was observed that apices cultured on solid MS media supplemented with 20μM BAP/1produced 3-8 shoots. Longitudinal splitting of shoots induced shoot clusters. Shoot elongation and rooting were obtained on media containing 1μM IBA/1 and 40gm/1 sugar (commercial). The regenerated plants were transferred to jiffy pots and kept in growth chamber for two weeks for acclimatization. Plants were then transplanted in field.