In vitro MUTAGEGENESIS IN OIL SEED BRASSICA

Abstract

Two Concentrations of EMS mutagens were used to induce the
genetic variations in microspore culture of two genotypes of oilseed rape (Brassica
napus). Embryos derived from mutated microspores were placed in liquid medium
NLN. Within a few days only surviving embryos were transferred to solid medium B5
for further development. The effects of various core of EMS concentrations on the
production and survival of embryos and plants in both genotypes has been discussed
and it was concluded that the lower concentration had the desirable mutagenic effects
for a practical mutation breeding programme of genetic improvement of oilseed
brassicas.