EVALUATION OF PHYTOCHEMICAL CONSTITUENTS AND ANTIBACTERIAL POTENTIAL OF AVICENNIA MARINA AND RHIZOPHORA MUCRONATA

Abstract

In the present study, phytoconstituents and antibacterial response of mangroves Avicennia marina and Rhizophora mucronata were evaluated. Various parts of both plants were collected from Indus delta, Thatta. Qualitatively test results confirmed that alkaloids, flavonoids, tannins, terpenoids, saponins and sterols are present in crude extracts of different parts of both plants. Total phenol contents, flavonoids and antioxidant activity were determined by spectrophotometer. Total phenol contents were varied from 10.70.5 to 29.851.2 mg/ml; total flavonoids from 2.370.42 to 16.530.19 mg/ml and antioxidants from 2.40.5 to 14.40.17 mg/ml in different parts of both plants. Leaf extracts of both mangrove species showed the highest amounts of total phenol, total flavonoids and antioxidant activity as 29.851.2 mg/ml, 16.530.19 mg/ml and 14.40.17 mg/ml respectively, while root extracts of both plants accumulated the lowest amount of total phenol, flavonoids and antioxidant activity. Crude ethanol extracts of A. marina and R. mucronata were also tested against three pathogenic bacterial species. T1 preparations (10% ethanol extracts) of leaves of both species showed a wide range of growth inhibitions 5.50.7 – 13.50.4 mm while T2 preparations (5% ethanol extract) showed poor response 3.00.65 - 7.60.2 mm against E. coli, K. pneumoniae, S. aureus