STUDY THE VIRULENCE FACTORS AND PATTERNS OF ANTIBIOTICS RESISTANCE IN ACINETOBACTER BAUMANNII ISOLATED FROM HOSPITALIZED PATIENTS IN BAGHDAD CITY

Abstract

Acinetobacter baumannii is an opportunistic nosocomiala multidrug resistance( MDR) pathogen, so
arising largely infections by this bacteria specially in immuno-compromised patients and ability to
survive in hospital environments and it became important human pathogen so , virulence factor and
antibiotic resistance are playing important role in infections but few studies in Iraq about this bacteria
there for present study aimed to study the virulence factors among Acinetobacter baumannii isolated
from Hospitalized patients among hospitals in Baghdad city and evaluate the antibiotics resistance in
Acinetobacter baumannii isolate.
Thirty-nine isolate of Acinetobacter baumannii were isolated during period March to October 2015 from
various clinical source from laboratories of bacteriology in different hospitals of Baghdad city then
diagnosis and identification by classical methods and vitek 2system, and study virulence factors as form
Biofilm; Capsule formation; Pellicle assay; hemolysin production and various enzymes so evaluated the
antimicrobial resistance for twelve different antibiotics.
Acinetobacter baumannii was more isolated from Wound and Burn swab(38.5%) so (28.2%) isolated
from both Urine and sputum , whilst (5.1%) form blood, and high percentage of Acinetobacter
baumannii (43.5%) in age group(40-60) years, whilst only (2.5%) in age less than 20 years, as well as
high percentage (59.4%) founded in males, Also biochemical test were positive for catalase and citrate,
while negative for each of Oxidase, indole , Urease , Lactose fermentation , motility and hemolysin ,
also all isolate were positive for gelatinase and 21 isolate positive for Protease whilst 29 isolate positive
for both Lipase and Capsule as well as 18 isolate positive for Lecithinase and 38, 33 isolate positive for
heamolysin production and Pellicle assay respectively . so 32 of Acinetobacter baumannii isolates were
positive for biofilm formation, also current study appearance all Acinetobacter baumannii isolates were
found resistant to ampicillin, Cefoxitin and tetracycline (100%), whilst low resistance to Imipenem and
Piperacillin 58.9, 15.4% respectively.
High percentage of Acinetobacter baumannii isolated from burn swab, whilst low percentage from
blood so high percentage isolated from age group (40-60) years and frommales patients more isolate
compared to females .as well as Acinetobacter baumannii isolates have multiple virulence factors that
apparent all Acinetobacter baumannii isolates have gelatinase activity whilst varied result other factors,
and highest resistance of isolates to Ampicillin, Cefoxitin and Tetracycline.