DNA FINGERPRINTING OF SOME HERBICIDE-TOLERANT STREPTOMYCES SPECIES AND PCR-ISOLATION OF BAR GENE
By Saleh, E.A.1 ; Zaki M.M.1 ; Rahal, A.2 ; Mohamed Sonya H.2,4; Bahieldin A.M.3 and A.S. Sadik1,3,4
In this study, differentiation between five identified Streptomyces species was carried out on the basis of the nucleotide sequence of DNA using random amplified polymorphisms of DNApolymerase chain reaction (RAPD-PCR) technique. Results revealed that these species differed in their DNA sequence as indexed by differences in numbers, sizes and unique amplified fragments. Such dissimilarities could be considered as an additional proof that identified isolates were different species of Streptomyces. In addition, the herbicide resistance (bar) gene was isolated by PCR technique in the DNA extracts of the five identified Streptomyces isolates (Streptomyces albosporeus subsp abilomycaticus, S. herbaricolor and S. aureomonopodiales) to be available for using as a selectable marker in the transformation of the transgenic plants using the Basta herbicide. It is the first evidence to detect this gene in local Streptomyces species in Egypt.
CHARACTERIZATION OF STREPTOMYCETES HAVING ANTIBIOSIS ACTIVITIES ISOLATED FROM SOIL IN WESTERN REGION OF KSA
By Shori Ghadeer B.O.1 , Mohamed Sonya H.1,2, Abdel-Salam Shimaa M.3 and Sadik A.S.1,4
In this study we are focused on the color groups of actinomycetes, in particularly streptomyctes, in soil of western region of KSA. Therefore, we collected soil samples from different climate locations in KSA (Taif, Makah and Jeddah).The color groups of the isolated actinomycete isolates were determined. The antagonistic activities of the isolated actinomycetes were also tested against seven microorganisms including, bacteria and fungi. The highest active isolates were identified as strains of S. polychromogenes (isolate 08), S. chattanoogensis (isolate 14), S. lucensis (isolate 20), S. violaceus (isolate 21), S. violans (isolate 32), S. griseorubiginosus (isolate 34), and S. antibioticus (isolate 35). It was show that the 7 selected streptomycete isolates were able to grow in the presence of 7% NaCl in the starch nitrate agar medium. At concentration of 10.5% NaCl, four isolates grew with weak growth (+) and three isolates showed in-doubt growth (±). Keywords: Actinomycetes, Streptomyces, Identification, Taif, KSA
EFFECT OF SODIUM AZIDE ON THE GROWTH OF CAPSICUM ANNUUM (CHILI)
By M. Umar Dahot, M. Rafiq, Ambreen M. Arif and S. H. Ahmed Naqvi
The present study was carried out to check the mutagenic effects of 0.5, 1.0 and 2.0% NaN3 on the germination and growth of Capsicum annum (Chili). The results showed that 0.5% NaN3 was most appropriate for the creation of mutagenesis and was not highly lethal. The higher germination rate was observed in control and 0.5% NaN3 treated seeds but germination rate was decreased in 1.0% and 2.0% NaN3 treatment. The variation was observed in plant fresh and dry weight, total and reducing sugars and total protein contents of plants in different concentrations of NaN3 treatments.
Isolation and Identification of some halotolerant Actinomycetes having antagonistic activities against some plant pathogens (i.e., Tobacco mosaic virus, Aspergillus Sp., Fusarium Sp.) from soil of Tai
By Mohamed Sonya H.1 , W.M.Omran 1 , M.Abdel-Salam Shimaa2 , A.S.Al-Shehri1 and A.S.Sadik
Actinomycetes are considered one of the important bacteria due to their ability to produce several substances as secondary metabolites, found to be effective in the control of some plant pathogens. In this study, a number of soil samples were collected from different locations of Taif as well as Jeddah and Makah. This was followed by determination of the microbial total counts, i.e., bacteria, fungi and actinomycetes. Actinomycetes were isolated and purified and their color groups were determined. The actinomycete isolates were identified based on their cultural and morphological properties as Streptomycetes. The salt tolerance range of the purified Streptomyces isolates were determined by growing them on starch nitrate agar medium supplemented with different NaCl concentrations ranged from 3.5 to 10.5%. The highly tolerant streptomycete isolates were grown on a starch nitrate broth medium for 6 days under shaking conditions at 28-30oC. Then, their antiviral and antifungal activities against TMV, Aspergillus sp. and Fusarium sp. were determined. The halotolerant Streptomycete isolates having antifungal and/or antiviral activities were completely identified based on their cultural, morphological and physiological properties. The DNA of the identified isolates was extracted and used for determination of DNA fingerprinting of these isolates using the RAPD-PCR molecular tool.