EFFECTS OF DIFFERENT PHYTOHORMONES ON SUGARCANE (SACCHARUM SPP.) REGENERATION
By Imtiaz Ahmed Khan+ , Abdullah. Khatri, Saboohi Raza, Ghulam Shah Nizamani,Muhammad Aquil Siddiqui, Nighat Seema, *Muhammad Umar Dahot and Mazhar H. Naqvi
Three sugarcane clones, AEC82-223, Ghulabi-95 and AEC86-328 were investigated for callus induction and regeneration under different concentrations (2mg/L and 4mg/L) of various auxins i.e. 2,4-dichlorophenoxyacetic acid (2,4-D), indol acetic acid (IAA), 3,6- dichloro 2-methoxy benzoic acid (Dicamba) and picloram. An efficient callus induction was observed on the medium containing picloram followed by 2,4-D but high callus proliferation was achieved with picloram. Non-regenerable callus was produced when calli were transferred for proliferation on Dicamba. The maximum calli induction, its proliferation and plantlets regeneration were recorded in clone AEC82-223, while the minimum in AEC86- 328. The maximum chlorophyll mutation frequency was noted in clone AEC86-328 and minimum in AEC82-223. Among auxin especially 2,4-D induces more genetic variability as compared to other auxins used in this work.
DOUBLED-HAPLOID PRODUCTION TECHNOLOGY IN WHEAT (TRITICUM AESTIVUM L.): ANTHER CULTURE
By M. A. SIAL, MAZHAR H. NAQVI, M. U. DAHOT*, M. A. ARAIN, S. M. MANGRIO** AND A. M. SOOMRO
To achieve homozygosity within the shortest possible period, doubled-haploid technology is a valuable technique to reduce the time and cost of development of new wheat varieties. It involves in vitro development of fixed lines from the parental material; development of each generation of progeny can be initiated before the parents have achieved physiological maturity. The exploitation of recessive genes can be rapidly achieved by microspore culture and anther culture via induction of sporophytic pathway from a gametophytic pollen pathway, resulting in callus or embryoids formation. Anther culture studies were conducted to produce haploid plants in five bread wheat genotypes BW-2, BW-5, BW-8, BW-9, and BW-16. Two media liquid and agar-solidified with different compositions were used to determine their effects on the induction of calli and regeneration of green plants. The potential of callus induction was significantly higher (82.4) in liquid culture medium as compared to agar-solidified medium (55.7) worked out on 100 anther basis. In liquid media, calli induction ranged from 37.8% to 117%, whereas, calli induction ranged from 39.1 to 73.6% on agar-solidified medium. The callus induction and plant regeneration were varied among genotypes on both the media. Genotype BW-8 was more conducive for callus induction to agar medium as compared to liquid medium, however, BW-9 showed less callus differentiation ability on both the media.
DIVERSITY AND SEASONAL OCCURRENCE OF PLANKTONIC ROTIFERS IN KEENJHAR LAKE DISTRICT THATTA SINDH PAKISTAN
By G.A. SAHATO AND K.H. LASHARI
Qualitative and quantitative Zooplankton sampling was carried out every month from November 1998 to October 1999 at three stations in Keenjhar Lake. A total of fifteen rotifer species were identified. Four species belonging to genus Keratella were K. cochlearis, K. volga, K. cochlearis var. tecta, Keratella tropica and four species belonging to genus Brachionus were B. falcatus, B.buidapestinensas, B.quadridentatus and Brubens. The other were Platyias quadriconus, Monostyla sp, Mytilina sp, Lecane sp, Tetramarti opotiensis, Euchlanis sp and Macrochaetis sp, Keratella and Brachionus were present through out the year.
A. W. BALOCH, A.A. RIND AND K. D. JAMALI
By A. W. BALOCH, A.A. RIND AND K. D. JAMALI
Rice is an important cereal crop of Asian countries. It is a major staple food crop Southeast and eastern countries of Asian sub-continent. Knowledge on the genetic constitution of rice plant has immense importance in Plant breeding Programmes. The use of molecular markers has facilitated the selection process. Markers-assisted selection has provided a reliable source for identifying and selecting the desirable genotype in plant breeding programmes. Molecular marker can save time and labour. It is a laborious job to grow a large number of F2 populations and practice selection for morphological markers in conventional plant breeding. Molecular markers will be more useful for selection when (1) the phenotype is difficult or expensive to measure directly, (2) genes of similar phenotype are being pyramided into a single line, or (3) markers are being used to select against the donor genome in a backcrossing programme.
STUDIES ON IN VITRO SURFACE STERILIZATION AND ANTIOXIDANTS ON GUAVA SHOOT TIPS AND NODAL EXPLANTS
By Roshan Zamir+, Syed Tariq Shah, *Nawab Ali, G.S.S. Khattak and T. Muhammad
In vitro studies were carried out to investigate the effect of different surface sterilization agents and antioxidants on the guava (Psidium guajava L.) Cv. Safeda shoots tips and nodal explants. The best sterilant observed for shoot tips was mercuric chloride (HgCl2) at 0.05% for 5 minutes plus 70% ethanol which gave maximum survival percentage of (67%) while minimum were survival (22%) was observed with 2% Sodium hypochlorite for 10 minutes. In case of nodal explants the best sterilant was 4% Calcium Hypochlorite (CaOCl2) for 10 minutes which gave 25% survival. Blackening of media was reduced and gave 55% green shoot tips when 75:50mg/l citric acid and ascorbic acid was supplemented to the medium. While 75:50 mg/l citric acid and ascorbic acid, 100mg/l Polyvinyl pyrolidone (PVP) and 200mg/l Activated charcoal when added to the medium gave 13% green nodal explants.
ISOLATION, CLONING AND PARTIAL CHARACTERIZATION OF THE GENE ENCODING THE POLYGALACTURONASE INHIBITING PROTEIN OF PHASEOLUS VULGARIS CV. NAZ
By A. HOSSAINZADEH COLAGAR*, M. MOTALLEBI and M.R. ZAMANI+
In this research, inhibitory effect of bean (Phaseolus vulgaris cv. Naz and cv. Derakhshan) hypocotyl PGIP on the PG enzymes from highly virulent isolates of Fusarium oxysporum (F15) and Ascochyta rabiei (IK04) was analyzed. It was shown that PGIPs from Naz and Derakhshan cultivars inhibit more effectively the PG activity of F. oxysporum (F15) than PG activity of A. rabiei (IK04). The inhibitory activity of Naz and Derakhshan PGIP on PG activity of these fungi demonstrated that Derakhshan PGIP was able to inhibit the PG activity of F. oxysporum by 55% and A. rabiei by 40%. Naz cultivar PGIP had reduced ability to inhibit PG activity from F. oxyspoum and A. rabiei by 40 and 26%, respectively. The pgip gene was amplified from Naz cultivar genomic DNA, cloned and sequenced. Comparison of DNA sequence and deduced amino acid from Naz cultivar with those of Derakhshan and Saxa cultivars indicates that it differs only in a single nucleotide (G) in position 880 which alters amino acid T to A at position 294. This amino acid is located in LRR9 region of PGIP. Also, the cloned pgip was shown to encode a 37 kDa polypeptide corresponding to the deduced polypeptide molecular weight. The data from this study suggestes possible role for this amino acid change in LRR9 region in specificity of PGIP-PG interaction.
Acknowledgment of Reviewers
|Prof. Dr. Richard M. Cooper||Bath, U.K.|
|Prof. Dr. Asif Ali||Faisalabad, Pakistan|
|Prof. Dr. J.J.Zhong||Shanghai, China|
|Prof. Dr. Jian He Xu||Shanghai, China|
|Prof. Dr. Muhammed Umar Dahot||Uni. Of Sindh, Jamshoro, Pakistan|
|Prof. Dr. Rafi Arain||Uni. Of Sindh, Jamshoro, Pakistan|
|Prof. Dr. Atta-u-Rehman||Uni. Of Sindh, Jamshoro, Pakistan|
|Prof. Dr. Rehmatullah Choudhary||Uni. Of Sindh, Jamshoro, Pakistan|
|Dr. Tayyab Hussain||Lahore, Pakistan|
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