About The Journal
The Pakistan Journal of Biotechnology (PJBt), a broad-based journal, is introduced to publish the most exciting research in all areas of basic and applied biotechnology and related fields. The Journal welcomes the submission of manuscripts that meet the general criteria of significance and scientific excellence. Read More
Current Issue 2020
Pages: 115-119 Vol-17 No-2
EVALUATING THE STATUS OF VITAMIN 25(OH) D LEVELS AMONG FEMALES OF ALL AGE GROUPS IN KARACHI, PAKISTAN (VITAMIN D LEVELS AMONG FEMALES IN KARACHI)Corresponding Author
Tehseen Fatima Zebtehseen.email@example.com,
Authors: 1Farrukh Abu Hazim, 2Rafat Amin, 2Urooj Ishrat, 4Neha Baqai, 2Tehseen Fatima Zeb*
Objective: Vitamin D plays an imperative role in growth, metabolism and reproduction. Low levels of this vitamin D, are highly prevalent globally and leads to various disorders besides different skeletal deformities. Women of different reproductive ages are more prone towards developing bone diseases as of low vitamin D levels. The study focuses to evaluate the prevalence of vitamin D deficiencies in females of different ages in Karachi, Pakistan.
Methods: Serum vitamin 25(OH) D levels of 1035 female were measured by electro-chemiluminiscence immuno assay. The data was analyzed through SPSS version 16.
Results: Among 1035 participants of different reproductive ages, 26.1% (n= 270) showed severe vitamin D deficiency, 44.2% (n=458) displayed mild to moderate Vitamin D deficiency while 29.7% (n=307) were having normal levels of serum vitamin D.
Discussion and conclusion: 70.4 % of our studied population had lower levels of vitamin D representing that majority of Pakistani women have vitamin D Deficiency. Since hypovitaminosis is a grim public health concern, strong recommendation of health education and vitamin D supplementations for the female population is proposed.
Keywords: Vitamin 25 (OH) D levels, Female population, different age, Karachi, hypovitaminosis
Pages: 107-113 Vol-17 No-2
AN OVERVIEW ON SUITABILITY OF ECO-FRIENDLY IPM APPROACH FOR MANAGEMENT OF BT-RESISTANCE IN PINK BOLLWORM ON COTTONCorresponding Author
Muhammad Shahid Iqbalshahidkooria@gmail.com
Authors: Rao Ahsan Ayaz1,2, Muhammad Rafiq Shahid1 , Saghir Ahmad1 , Muhammad Javaid2 and Muhammad Shahid Iqbal1*
Pink Bollworm (Pectinophora gossypiella) member of the family Gelechiidae is known to be among the most destructive insect pest of cotton, causing huge damage to the cotton seed as well as heavy loss in fiber quality of the crop. Its larvae are active feeder of the reproductive parts of cotton and conceal in the damaged bolls. Large scale cultivation of transgenic cotton that produces Cry1Ac resulted in development of resistance in pink bollworm against Cry1Ac. A single control tactic even chemical control remains ineffective for their management and all possible tactics are integrated for their effective control. For its monitoring Sex pheromone traps, PB Ropes and light traps are installed. Growing of non Bt refugia also play an effective role for the management of pink bollworm. Keeping in view, the importance of Integrated Pest Management (IPM) is an ecofriendly and effective technique as compared with the use of conventional insecticidal technique. /uploads/2020/Vol-17/No-2/PJBT-VOL-17-NO-2-OF-YEAR-2020 (8).pdf
Keywords: Pectinophora gossypiella: Bt resistance: Management Strategies
Pages: 101-105 Vol-17 No-2
PUBLIC AWARENESS INFORMATION ON HUMAN VIRUSES AND CORONAVIRUS (COVID-19): I. CHARACTERISTICS AND PROTECTIONCorresponding Author
Atef Shoukry Sadikatef_sadik@yahoo.com & firstname.lastname@example.org
Authors: Atef Shoukry Sadik
This research can be considered as simple awareness information for the people with limited education in Egypt as well as the world in addition to specialists. It also allows decision makers in the World Health Organization and other countries to devise appropriate strategies to respond to any epidemic such as the Coronavirus and others that may arise in the future. This article has dealt with a simple definition of viruses and then focus on the characteristics of human viruses in a simplified manner, whether their ability to enter the family of the host, how to influence it, the concept of the incubation period, the role of the immune system in the long of this period, and the prevalence of an epidemic including viruses. This is in addition to the symptoms of coronavirus infection, and prevention methods. Finally, the study pays an attention on the most nutrition system and factors that positively affect the immune system and its strengthening in a way that allows humans to confront viral infection.
Keywords: Viruses, coronavirus infection, symptoms, incubation period, immune system, nutrition, controlling strategies.
Pages: 93-99 Vol-17 No-2
BIBLIOMETRIC ANALYSIS OF THE PAKISTAN JOURNAL OF BIOTECHNOLOGYCorresponding Author
Authors: Waseem Hassan a, , Haseeb Hassan b and Mehreen Zafar a
This is the first bibliometric report about the Pakistan journal of biotechnology (PJBt), which was launched in 2004. Total number of publications was found to be 825. Statistically, a random yearly growth rate was observed (varied per year) and the doubling time was found to be 4.7 (years). From 2008 to 2017, the data of 501 publications was retrieved from Scopus. Precisely, 1224 authors, 714 institutions and 29 countries were directly involved in (501) research publications. In author’s category, Prof. Dahot M.U. was found with highest number of publications i.e. sixteen (16). Institute of Biotechnology and Genetic Engineering, University of Sindh, Jamshoro, Pakistan is the top university with (29) publications. While, India is the top ranked country with maximum (271) number of publications. We also provided the list of top ten most cited documents. Last but not the least, the detail graphical maps are provided for the co-words analysis of titles, abstracts and all keywords of the manuscripts (Vosviewer analysis). This will significantly help to describe the overall theme of the journal.
Keywords: Scopus, PJBt and Bibliometry
Pages: 85-91 Vol-17 No-2
ANTIHYPERTENSIVE PEPTIDES PRODUCED BY INDIGENOUS LACTIC ACID BACTERIA FROM DADIH ORIGINCorresponding Author
Chandra Utami Wirawaticutami@polinela.ac.id
Authors: Chandra Utami Wirawati1*, Dwi Eva Nirmagustina and Yatim Rahayu Widodo
In this study the capability of Lactobacillus plantarum ssp. plantarum DG17 and Lactococcus lactis ssp. lactis DK12 isolated from dadih origin was considered to generate antihypertensive peptides in reconstituted skim milk (RSM) medium followed bymeasuringangiotensin converting enzyme (ACE) inhibitor activity, protein profile and protein sequence. Theisolates showed high ACE inhibitor abilities i.e. 60.79±6.2% and 61.98±7.8%, with IC50 values of 439.9 µg/mL and 442.2 µg/mL respectively. Two smaller molecular weight bands ranging in size from 12 to 14 kDa were recorded in peptides profile of these isolates and assumed as antihypertensive peptides. The peptides sequence with molecule weight less than 3.0kDa were identified by LC Nano/MS and then compared withthe peptide database (Milk Bioactive Peptide Databaseand BIOPEP-UWM).One of the peptide sequence (VVVPPF) generated from Lactobacillus plantarum ssp. plantarumDG17had high ACE inhibitor activity due to the presence of F residue at the C terminal and V residue at the N terminal. The experimental result indicated that Lactococcus lactis ssp. Lactis DK12 and Lactobacillus plantarum ssp. plantarum DG17 isolates were potential developed into a starter culture in functional fermented milk.
Keywords: : Lactobacillus plantarum, Lactococcus lactis, ACE inhibitor, antihypertensive peptides
Pages: 79-84 Vol-17 No-2
EVALUATION OF DIFFERENT FUNGICIDES, BOTANICAL EXTRACTS AND BIOCONTROL AGENTS AGAINST ALTERNARIA ALTERNATA THE CAUSAL AGENT OF LEAF SPOT IN SPINACHCorresponding Author
Manzoor Ali Abrommanzoorabro@hotmail.com
Authors: Aziz Ullah Kakar1 , Manzoor Ali Abro1* , Ghulam Hussain Jatoi1 , Mir Shahbaz Ali Talpur1 , Waseem Ali Soomro1 , Suman Qazi1 , Mohammad Dawood2 and Adnan Baloch2
Alternaria disease is among the most important diseases of Spinach throughout the world. They affect primarily the leaves. Alternaria spp. is major plant pathogens, which cause at least 20% of agricultural spoilage most severe losses may reach up to 80%.This study was carried out to study the in-vitro efficacy of different fungicides, botanical extracts and bio-control agent against leaf spot disease of spinach caused by Alternaria alternate. Cabrio top showed minimum mycelial colony growth (23, 21 and 17 mm) at various concentrations 100, 200 and 300 ppm, respectively, followed by Topsin-M (44.5, 33 and 19 mm), Melodyduo (33, 26.5 and 22.5 mm), Prevail (43.5, 37 and 22.5 mm) and Antracol (41, 33 and 24 mm) at various concentrations 100, 200 and 300 ppm, respectively. However, in control, the mycelial colony growth of A. alternata causing leaf spot disease was recorded as 90 mm at 100, 200 and 300 ppm concentrations. Minimum mycelia colony growth (43, 35 and 15 mm) was recorded at 5, 10 and 15% concentration for Aak followed by Garlic (43.5, 33 and 20 mm), Neem (49, 38.5 and 23 mm), Onion (57.4, 50.25 and 27.65 mm) and Eucalyptus (57.9, 51.4 and 33.45 mm) and maximum mycelial colony growth (90.00 mm) were recorded in control. Minimum mycelial colony growth of A. alternata was observed for Neurospora spp. (47.00 mm) followed by Hypocrea sp. (48.00 mm), Lasodiplodia sp. (49.5 mm), Fusarium sp. (57.5 mm) and Chactomium sp. (57.5 mm). Maximum mycelial colony growth (90 mm) was recorded in control.
Keywords: Alternaria alternate, Biocontrol, Botanical extracts, Fungicides, leaf spot, Spinach
Pages: 71-77 Vol-17 No-2
IDENTIFICATION OF VALUABLE TRAITS THROUGH MOLECULAR AND MORPHOLOGICAL MARKERS IN DIPLOID WHEATCorresponding Author
Authors: Zareen Sarfraz1* , Mohammad Maroof Shah1 , Muhammad Shahid Iqbal2 , Mian Faisal Nazir2 , Syeda Akash Fatima
Bread wheat (Triticum aestivum L.) harbors hexaploid genome with a complex of three diploid genomes (A, B and D). Major goals of today’s research in the wheat world count basically on improvement of grain quality and high yielding genotypes. It is relatively easier to target its diploid lineages for identification of high yielding genomic segments as compared to hexaploid with least polymorphism. The current study was aimed at the detection of genes/QTLs of high economic interest on ‘A’ genome of diploid progenitors. Segregating population from cross of Triticum monococcum and Triticum boeoticum was utilized for the purpose. 98 RILs along with parents were analyzed for various quantitative and qualitative traits. The molecular data obtained from RAPD markers was analyzed along with morphological traits data to identify association among them. Single factor ANOVA gave association (?= 0.01) of days to booting, days to heading and days to flowering with plant type and leaf orientation, flag leaf width with crown color, number of spikes per experimental unit with leaf orientation, 1000 kernel weight with aleurone color and RAPD-11 (locus b).
Keywords: A-genome donor, Wheat, RILs, RAPDs, QTLs
Pages: 63-70 Vol-17 No-2
EFFECT OF RATIO OF BEES WAX AND CARNAUBA WAX IN MIXED WAX ON RESPIRATION RATE, WEIGHT LOSS, FRUIT DECAY AND CHEMICAL QUALITY OF VIETNAMESE PASSION FRUITS DURING LOW TEMPERATURE STORAGECorresponding Author
Authors: Nguyen Sang*¹ and Le Ha Hai¹
This research aims at studying the impacts of ratio of bees wax and carnauba wax in mixed wax (MW) on weight loss, respiration rate, fruit decay and the chemical quality of the Vietnamese fresh purple passion fruit in storage period at low temperatures. Firstly, the MW1, MW2 and MW3 were made from bees wax and carnauba wax with 2 to 1, 1.5 to 1 and 1 to 1 ratios respectively at concentration of 8%. After that, passion fruits were coated in MW1, MW2 and MW3 for thirty seconds and dried for eight hours at room temperatures. After that, they were stored at 5±1°C for 42 days. The control was uncoated fruits. The rate of respiration, weight loss and fruit decay and total microorganism, chemical quality, content of total soluble solids (TSS), total titratable acidity and content of vitamin C were monitored during the storage period. According to the results, the MW1 treatment had the lowest rate of respiration rate, fruit weight loss, fruit decay and total microorganisms for a 42 day duration in storage. Besides, this control and all treatments could preserve the chemical quality of the passion fruit in terms of TSS content, total titratable acidity and content of vitamin C during storage time with insignificant difference (P?0.05).
Keywords: Purple passion fruit, bees wax, carnauba wax, mixed wax coating, respiration rate, weight loss, fruit decay.